A preparation method and application of a chloramphenicol antigen

摘要

#$%^&*AU2020101542A420200903.pdf#####ABSTRACT The invention discloses a method for preparing chloramphenicol antigen and an application thereof, belonging to the field of biotechnology, which comprises the following steps: Preparing chloromycetin antigen, utilizing specific immunological reaction of antigen and antibody. In this invention, the preparation method is simple and feasible, the cost is low, and the semi-antigen yield is high, and the prior detection technology overcomes the defects such as the complex and time-consuming pre-treatment of the chloramphenicol sample, a large amount of organic solvent for extraction and ssophisticated and expensive testing equipment used in the testing process and is not suitable for general use. In the invention, the chloramphenicol antigen of the present invention can produce specific antibody against the chloromycetin by immunize animals, which is for the rapid detection of chloramphenicol residues in food, and has many advantages, such as simple operation, fast, large amount of sample processing, high sensitivity, strong specificity.