METHOD FOR SEPARATING AND PURIFYING LOW-IRON SATURATION RECOMBINANT HUMAN LACTOFERRIN FROM GENETICALLY ENGINEERED RICE SEEDS

摘要

Provided by the present invention is a method for separating and purifying low-iron saturation recombinant human lactoferrin from genetically engineered rice seeds: first, extracting a crude extract containing recombinant lactoferrin from a recombinant human lactoferrin genetically engineered rice seed, wherein an extraction buffer solution contains 50-100 mM citric acid-trisodium citrate, 100-200 mM NaCl, 1-10 mM EDTA-2Na, and the pH thereof is 3.8-5.0; next, performing cation chromatography on the obtained crude extract containing the recombinant human lactoferrin to obtain a low-iron saturation lactoferrin product having a purity95%. Further provided by the present invention is a method for extracting low-iron saturation recombinant human lactoferrin from genetically engineered rice seeds, wherein the used extraction solution contains citric acid-trisodium citrate and EDTA-2Na, which may significantly reduce the iron saturation of the recombinant human lactoferrin.