Effects of sugar concentration on recombinant human #alpha#_1-antitrypsin production by genetically engineered rice cell

摘要

Productivity of recombinant human ~i -antitrypsin (rAAT) with a genetically engineered rice ccii using an inducible promoter has been studied by batch-wise and continuous production. A simple model explained the effect of ‘rore.~ses released from the disrupted cells on the rAAT degradation. Glucose concentration in the medium significantly affected the rAAT productivity in the continuous production. because the rAAT was induced by sugar depletion. When the fresh medium contain:ne 5 mM glucose was supplied to the continuous bioreactor, induction time was long and the productivity was low, indicatine that the clucos.e concentration in the cells was hiTh enouoh as to repress the promoter. When the glucose concentration in the fresh medium was reduced to 0.5 mM. total amount of rAAT produced in 70h cultivation reached 6.7—7.6mglg-dry cell, which was two times larger than the control medium without clucose.