FE-S FUSION PROTEIN ACTING AS ELECTRON TRANSFER CHAIN, NOVEL CARBON MONOXIDE FORMATE REDOX ENZYME MEDIATED THROUGH FES FUSION PROTEIN, NOVEL STRAIN BCF12 DERIVED FROM THERMOCOCCUS WHEREIN ENZYME IS TRANSFORMED, AND USE THEREOF

摘要

The present invention relates to a Fe-S fusion protein acting as an electron transfer chain, a novel carbon monoxide formate redox enzyme mediated through a FeS fusion protein, a novel strain BCF12 derived from Thermococcus wherein an enzyme is transformed, and use thereof. The Fe-S fusion protein of the present invention, by mediating itself, physically directly binds two different enzymes, so that electrons generated in either of the enzymes can be directly transferred to the other enzyme through a Fe-S cluster of the Fe-S fusion protein. Thus, by directly supplying the electrons necessary for the production of a target substance, a high-efficiency production reaction of the target substance without leakage of electrons generated by either of the enzymes is possible. In addition, the present invention has the advantages that the overall enzyme reaction rate and yield can be significantly improved by using a new electron transfer reaction that did not previously exist, and the stability of each enzyme can also be ensured by allowing the enzymes to be physically fixed within a cell.