ZEA MAYS NLP TRANSCRIPTION FACTOR ZMNLP5 AND USE THEREOF

摘要

The present invention involves cloning a gene ZmNLP5 from Zea mays that plays an important regulatory role in nitrogen nutrition assimilation and utilization, wherein the open reading frame of the gene has the DNA sequence as shown in SEQ ID NO: 1. A transcription factor protein encoded by the ZmNLP5 gene has the amino acid sequence as shown in SEQ ID NO: 2. Also provided are the uses of the above-mentioned Zea mays NLP transcription factor ZmNLP5 in promoting the expression of a key enzyme gene ZmNIR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNIR1.2 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.1 for nitrogen metabolism, in promoting the expression of a key enzyme gene ZmNR1.2 for nitrogen metabolism, in improving the nitrogen nutrition assimilation and utilization by Zea mays, and in promoting the elongation and growth of corn roots in low nitrogen environments. The Zea mays NLP transcription factor ZmNLP5 of the present invention can promote the expression of key enzyme genes for nitrogen metabolism, improve the nitrogen nutrition assimilation and utilization by Zea mays, and promote the elongation and growth of corn roots in low nitrogen environments, and is suitable for large-scale popularization and application.