BACILLUS LICHENIFORMIS (B.LICHENIFORMIS) ENGINEERING BACTERIUM CAPABLE OF RESTRAINING AUTOLYSIS OF THALLI AND CONSTRUCTION METHOD AND APPLICATION FOR B.LICHENIFORMIS ENGINEERING BACTERIUM

摘要

Provided are a B.licheniformis engineering bacterium capable of restraining autolysis of thalli and a construction method and application for the B.licheniformis engineering bacterium. The B.licheniformis engineering bacterium capable of restraining the autolysis of thalli is obtained by knocking out a pcf gene, and the nucleotide sequence of the pcf gene is as shown in SEQ ID NO.1. According to the B.licheniformis engineering bacterium capable of restraining the autolysis of thalli, the biomass is significantly increased; compared with the unmodified B.licheniformis, the autolysis rate is reduced by 11.2%, and fermentation production of α-amylase can be improved.