B.licheniformis engineering bacteria for inhibiting bacterial autolysis, construction method and application thereof

摘要

#$%^&*AU2020204574A120200806.pdf#####Abstract Disclosed are B.lichenformis engineering bacteria for inhibiting bacterial autolysis, and a construction method and application thereof, belonging to the field of microbial strain transformation. The B.lichenformis engineering bacteria for inhibiting bacterial autolysis are obtained by knocking out pcf genes, and the nucleotide sequence of the pcf genes is shown as SEQ ID No. 1. The biomass of the prepared B.lichenformis engineering bacteria for inhibiting bacterial autolysis is obviously improved, the autolysis rate is reduced by 11.2% compared with unmodified B.ichenformis, the autolysis of the B.lichenformis bacteria is reduced to a certain extent, the fermentation production of a target enzyme can be effectively improved, and the engineering bacteria have good application prospect.