PREPARATION AND EXPANSION CULTURE OF ENDOTHELIAL PROGENITOR CELL

摘要

The present invention addresses the problem of providing a means for easily preparing endothelial progenitor cells at high purity and low cost. The present invention also addresses the problem of providing a method for efficiently proliferating endothelial progenitor cells. Endothelial progenitor cells are prepared at high purity by the steps of: differentiating pluripotent stem cells into endothelial progenitor cells; and purifying the endothelial progenitor cells using the difference in adhesion ability between the endothelial progenitor cells of the cell population obtained in the previous step and other cells. Meanwhile, the endothelial progenitor cells are cultured and proliferated in the presence of a ROCK inhibitor, a GSK-3β inhibitor, and a TGF-β receptor inhibitor as well as a basic fibroblast growth factor and an epidermal growth factor.