A culture system (100) is provided with: a culture vessel (3) in which cells are to be cultured; a separation vessel (4) in which a liquid culture (30) is separated into a supernatant (41) and a precipitated liquid (42) containing cells by gravitational sedimentation; a first connecting passage (1A) and a second connecting passage (1B) through which the culture vessel (3) and the separation vessel (4) are connected to each other; a collecting passage (2) which is connected to the separation vessel (4) to collect the supernatant (41); and a control section (7) for controlling the flow of each of the liquid culture (30), the supernatant (41) and the precipitated liquid (42). The control section (7) is so configured that the liquid culture (30) can flow from the culture vessel (3) into the separation vessel (4) through the first connecting passage (1A), the supernatant (41) can flow out from the separation vessel (4) through the collecting passage (2) after the liquid culture (30) is allowed to stand, and subsequently the precipitated liquid (42) can be returned from the separation vessel (4) to the culture vessel (3) through the second connecting passage (1B).
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