METHOD OF DNA BASE-CALLING FROM A NANOCHANNEL DNA SEQUENCER

摘要

A method of DNA base-calling from a nanochannel DNA sequencer. The method includes building a reference map and preparing an unknown sequence of DNA prior to the final step of data matching. The reference map includes a series of reference characters, such as numbers, that describe the change in tunneling current of a DNA strand with a known sequence. A DNA strand of unknown sequence is prepared so that the change in electrical measurement can also be described numerically. The section of match between the DNA strand of unknown sequence and the reference map is used to determine the sequence of the DNA strand.