POINT-OF-CARE REAL-TIME GENE TESTING KIT AND DIAGNOSIS SYSTEM FOR PLANT DISEASE

摘要

The present invention is to provide diagnostic primers for cucumber mosaic virus, pepper mild mottle virus, and zucchini yellow mosaic virus, and a method for diagnosis of plant diseases by using same. Disclosed according to the present invention is a method for diagnosis of a plant disease, the method comprising the processes of: extracting a ribonucleic acid template by separating a plant specimen, immersing same in RTU RNA buffer in a tube, grinding same with a micro pestle, incubating and dissolving same for 10-15 minutes at room temperature, taking the supernatant from the tube, and diluting the supernatant; obtaining a PCR product by incubating the ribonucleic acid template with a reverse transcriptase at 36-37℃ for 9-11 minutes to generate deoxyribonucleic acid (DNA), introducing the DNA, together with species-specific primers utilizing plant gene nucleotide sequences, an enzyme, and buffer, into a polymer chip, and performing a polymerase chain reaction (PCR) with a portable real-time PCR instrument; and analyzing the PCR product to determine the presence or absence of pathogens in the specimen and detecting the pathogens.