ZIPPER FASTENER STRUCTURE OF PROMOTING FORMATION OF PROTEIN DIMER AND APPLICATION THEREOF

摘要

The present invention relates to the field of genetic engineering, and provides a zipper fastener structure of promoting formation of a protein dimer and application thereof. The zipper fastener can be applied to dimerization of proteins of the same type and dimerization of proteins of different types, and can also be applied to polypeptide cycle formation, polypeptide dimerization, and polypeptide extension. A ESAT6-CFP10 dimer having an approximately native conformation can be obtained, and the dimer has better solubility, and has a better stimulating effect on memory T cells than a ESAT6-CFP10 protein capable of linear fusion expression. A dimer zipper fastener can assist the formation of a more stable cyclic polypeptide, and a CCP polypeptide added with a dimer fastener can improve the detection rate for citrullinated autoantibodies in serum of a rheumatoid arthritis patient.