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GEMINIVIRAL VECTORS THAT REDUCE CELL DEATH AND ENHANCE EXPRESSION OF BIOPHARMACEUTICAL PROTEINS
GEMINIVIRAL VECTORS THAT REDUCE CELL DEATH AND ENHANCE EXPRESSION OF BIOPHARMACEUTICAL PROTEINS
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机译:减少细胞死亡并增强生物制药蛋白表达的妇科病毒载体
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摘要
The disclosure relates to a T-DNA binary vector based on bean yellow dwarf virus (BeYDV) that reduces plant cell death and increases transgene expression. In one aspect, the T-DNA region comprise a replicon cassette comprising a rep gene or a repA gene with a mutated translation initiation region. The disclosure also relates to replicating geminiviral expression system based on BeYDV comprising with an expression cassette a sequence encoding Rep and a sequence encoding the promoter of ubiquitin-3 from potato with ubiquitin fusion; an expression cassette comprising a sequence encoding RepA and a sequence encoding the promoter of ubiquitin-3 from potato with ubiquitin fusion; and an expression cassette comprising a promoter region, a 5' UTR, a sequence encoding a recombinant protein, and a 3' UTR. These expression cassettes are on different T-DNA cloning vectors or on one T-DNA cloning vector.
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