RNA METHOD FOR EXPRESSION OF HETEROLOGOUS PROTEINS USING A RECOMBINANT NEGATIVE-STRAND RNA VIRUS VECTOR

摘要

The present invention comprises the step of introducing one or more heterologous nucleic acid sequences into a cell by infecting the cell with a (-) strand of a recombinant RNA viral vector comprising one or more heterologous nucleic acid sequences, wherein the (-) strand of the Recombinant RNA viral vectors contain a viral genome that encodes the mutated P protein, resulting in loss of viral transcriptional ability without loss of viral genome replication, and the ability of the one or more heterologous nucleic acid sequences to reprogram the cell. Or a method for expressing one or more heterologous nucleic acid sequences in a cell, which encodes a therapeutic protein. In addition, the present invention provides a pharmaceutical composition comprising the cell or cell population as well as a cell or cell population prepared in vitro by the above method.