首页>
外国专利>
Method for expression of heterologous proteins using a recombinant negative-strand RNA virus vector comprising a mutated P protein
Method for expression of heterologous proteins using a recombinant negative-strand RNA virus vector comprising a mutated P protein
展开▼
机译:使用包含突变的P蛋白的重组负链RNA病毒载体表达异源蛋白的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a method of expressing at least one heterologous nucleic acid sequence in a cell, the method comprising introducing at least one heterologous nucleic acid sequence into a cell by infecting said cell with a recombinant negative-strand RNA virus vector comprising said at least one heterologous nucleic acid sequence, wherein the recombinant negative-strand RNA virus vector includes a viral genome coding for a mutated P protein, which leads to a loss of the viral genome replication ability without a loss of the viral transcription ability, and wherein said at least one heterologous nucleic acid sequence encodes a cellular reprogramming or programming factor or a therapeutic protein. In addition, the present invention provides a cell or a population of cells prepared in vitro by said method as well as a pharmaceutical composition comprising said cell or population of cells.
展开▼