Adapter-based retroviral vector system for selective transduction of target cells

摘要

The present invention provides a composition comprising: i) a pseudotyped retroviral vector particle comprising the following or a virus-like particle thereof: a) a kind of envelope protein having antigen-binding activity, wherein the envelope protein is It is a recombinant protein that does not interact with at least one of its native receptor(s), and is fused to a polypeptide comprising an antigen binding domain specific for the tag of the polypeptide tagged in its ectodomain, wherein the envelope protein is paramyxobiotic A protein of G, HN or H derived from the family Lydae, and b) a kind of envelope protein having a fusion activity derived from the Paramyxoviridae family, and ii) the tagged polypeptide, wherein the tag is attached The tagged polypeptide is a polypeptide that is specifically bound to an antigen expressed on the surface of a target cell, thereby transducing the target cell with the retroviral vector particle or inducing uptake of the virus-like particle into the target cell. . Also disclosed is a pharmaceutical composition and an in vitro method for transduction of target cells with the vector particles.