How to rescue a stop codon through gene redirection by ACE-tRNA

摘要

In certain embodiments, the invention provides a modified delivery RNA comprising a T-arm, a D-arm, and an anticodon-arm and a recipient arm, wherein the T-arm comprises a nucleotide that interacts with the renal factor 1 alpha protein. (tRNA), and methods of using the same. In certain embodiments, the present invention provides a modified delivery RNA (tRNA) comprising a T-arm, a D-arm, and an anticodon-arm and a recipient arm, (a) an anticodon-arm comprising a tri-nucleotide anticodon And the anticodon is 5'-UCA-3' and recognizes the TGA stop codon, and the acceptor arm is operably linked to arginine, tryptophan or glycine; (b) the anticodon-arm comprises a tri-nucleotide anticodon, the anticodon is 5'-UUA-3' and recognizes the TAA stop codon, and the acceptor arm is operably linked to glutamine or glutamate; Or (c) the anticodon-arm comprises a tri-nucleotide anticodon, the anticodon is 5'-CUA-3' and recognizes the TAG stop codon, and the recipient arm is operably linked to tryptophan, glutamate or glutamine.