Modified ligand-gated ion channels and methods of use

摘要

This disclosure relates to materials and methods for modulating ligand gating ion channel (LGIC) activity. For example, a modified LGIC is provided comprising at least one LGIC subunit having a modified ligand binding domain (LBD) and/or a modified ion pore domain (IPD). In addition, exogenous LGIC ligands capable of binding and activating modified LGIC, as well as methods of modulating ion transport across the membranes of mammalian cells, methods of modulating the excitability of cells in mammals, and mammals with channel disease Methods of treating animals are provided.