The present invention provides a nucleic acid sequence comprising a sequence of a contiguous nucleotide, the sequence of the contiguous nucleotide has at least 90% sequence identity with the nucleic acid sequence of SEQ ID NO: 1, and the sequence of the contiguous nucleotide is single-chain It encodes the BoNT/E1 protein. The present invention also provides a method for producing a soluble single-chain BoNT/E1 protein in an E. coli host cell, together with a method for producing a soluble di-chain BoNT/E1 protein.
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机译：本发明提供了包含连续核苷酸的序列的核酸序列，该连续核苷酸的序列与SEQ ID NO：1的核酸序列具有至少90％的序列同一性，并且该连续核苷酸的序列是单个的-链编码BoNT / E1蛋白。本发明还提供了一种在中产生可溶性单链BoNT / E1蛋白的方法。大肠杆菌宿主细胞，以及生产可溶性双链BoNT / E1蛋白的方法。
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