Method for pathogen enrichment and nucleic acid extract using device for point of care testing

摘要

The present invention relates to a method for concentrating pathogens and extracting nucleic acids using dimethyl subverimidate as a diatomaceous earth functionalized with an amine and a crosslinking agent, the pathogen having a negative charge is electrically absorbed by the surface of the diatomaceous earth positively charged by the crosslinking agent, The nucleic acid released after cell lysis can be fixed and separated on diatomaceous earth through reversible crosslinking with amine groups of diatomaceous earth. The method can be used as an on-site diagnostic method by simultaneously concentrating pathogens and extracting nucleic acids with an integrated device without the use of special equipment and electricity (electric power), and is superior in time and cost savings than the conventional method, and the extracted nucleic acid is a disease There is an advantage that can be useful in the diagnosis and treatment of.