Composition of serum-free medium containing human stem cell-derived exosomes for cell culture

摘要

The present invention provides a new serum-free medium composition for cell culture in which exosomes that induce cell adhesion and proliferation are separated from human stem cells, and exosomes are contained in a basic medium. The serum-free medium composition for cell culture of the present invention contains genes, proteins, growth factors, etc. related to the growth of cells, so it can aid in the adhesion and growth of cells without other additives such as cell activators or growth factors, and prevent cell proliferation. Induction can be promoted. The human stem cell-derived exosome used in the present invention is a purified component that does not contain harmful factors of antibiotics, serum, or culture medium, so it can overcome the problems of animal serum, and because it is a cell-derived lipid carrier, it is a cell penetration and effective factor. The transfer efficiency is very good. Therefore, the present invention can minimize the disadvantages of serum by using a medium to which exosomes are added instead of animal serum or by reducing the amount of serum to a minimum, and finally can be used for culturing stem cells for cell therapy for clinical application. have.