ENVIRONMENTS AND METHODS OF FERMENTATION FOR THE PRODUCTION OF POLYSACCHARIDES IN BACTERIAL CELL CULTURE

摘要

The present invention relates to media and fermentation methods for producing polysaccharides in bacterial cell culture. In one aspect, the invention relates to a complex culture medium comprising a vegetable hydrolysate, a yeast extract, and a carbon source. In another aspect, the invention relates to a defined media having a total amino acid concentration greater than about 50 mM. A further aspect of the invention relates to the use of fed batch and perfusion fermentation methods for cultivating polysaccharide-producing bacteria.