METHOD OF SIMULATING AN INFECTED WOUND IN RATS WITH BACKGROUND OF LONG-TERM CURRENT DIABETES MELLITUS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely to experimental surgery, and can be used for simulating an infected wound with background of long-term current diabetes in experiment. Alloxan 200 mg/kg is administered on an empty stomach, 15 minutes after alloxan introduction, remaxol solution is administered intraperitoneally 13 ml/kg. Then, in 93 days, skin is excised in an inter-blade area to a superficial fascia in the form of a circle with diameter of 40 mm on the surface of the bottom of the formed wound, and the superficial fascia is dissected by transverse and longitudinal mutually perpendicular incisions. A purse-string suture is applied along the wound edges; the suture is tightened to bring the wound up to 20 mm in diameter; skin-facial nodal sutures are applied. Then, a gauze swab with a suspension of a daily culture of a polyantibiotic-resistant clinical strain of Staphylococcus aureus in dose of 2 billion microbial bodies in 1 ml of physiologic saline is introduced into the wound.;EFFECT: method enables simulating an infected wound with a background of long-term current diabetes mellitus with all the morphological features characteristic of it by simulating long-term current diabetes mellitus by an alloxan model of diabetes mellitus and using the remaxol preparation possessing high hepatoprotective and antioxidant action, and by simulating the infected wound by introducing a Staphylococcus aureus into the wound.;1 cl, 4 dwg, 2 tbl, 3 ex