METHOD OF CULTIVATION OF MICROALGAE RHODOMONAS SALINA

摘要

FIELD: biotechnology.;SUBSTANCE: invention represents a method for cultivation of microalgae Rhodomonas salina, including cultivation of algae in cultivators on nutrient medium at temperature and illumination and obtaining of biomass, algae are cultivated for 7–8 days by accumulation culture method at temperature 24±1 °C under conditions of round-the-clock illumination with illumination of 10 klx, with continuous bubbling with air. As a nutrient medium Conway's medium is used, modified by increasing concentration of iron in 2 times, prepared on the basis of sterile sea water with salinity of 18 ‰ with addition of the following macroelements, g/l: NaNO₃ – 100.0; NaH₂PO₄ ⋅ 2H₂O – 20.0; FeCl₃ ⋅ 6H₂O – 5.2; Na₂EDTA – 45.0; H₃BO₃ – 33.6; MnCl₂ ⋅ 4H₂O – 0.36 and microelements, g/100 ml: ZnCl₂ – 2.1; CoCl₂ ⋅ 6H₂O – 2.0; CuSO₄ ⋅ 5H₂O – 2.0; (NH₄)₆Mo₇O₂₄ ⋅ 4H₂O – 0.9; H₂O dist. – 100.0.;EFFECT: obtaining a complete fodder and in sufficient quantity for larvae and molluscs spat grown in the nursery.;1 cl, 3 dwg, 2 tbl