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METHOD FOR DIAGNOSING CHRONIC ENDOMETRITIS IN FEMALES WITH ENDOMETRIOSIS

机译:诊断子宫内膜异位症的慢性子宫内膜异位症的方法

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, particularly to pathological anatomy and gynecology, and aims at diagnosing chronic endometritis in females with endometriosis. Endometrium pipelle-biopsy is performed in middle stage of proliferation phase or menstrual cycle secretion phase. Obtained biomaterial is immunohistochemically studied by staining the slits using a monoclonal antibody – Bond Ready-to-Use Primary Antibody CD68. Amount of CD68 macrophages is determined by counting the number of positive-colored cells by 1 mm2. If the number of positive-stained CD68 cells of endometrium is 1 mm2 at the middle stage of proliferation from 151 to 293 or in the middle stage of the secretion phase from 425 to 523, chronic endometritis is diagnosed. If the number of positive-stained CD68 cells of endometrium is 1 mm2 at the middle stage of the proliferation phase from 49 to 150 or in the middle stage of the secretion phase from 74 to 424, this corresponds to the norm.;EFFECT: invention provides effective diagnosis of chronic endometritis in females with endometriosis.;1 cl, 3 dwg, 4 ex
机译:技术领域本发明涉及医学,特别是病理解剖学和妇科,旨在诊断患有子宫内膜异位的女性的慢性子宫内膜炎。子宫内膜移行活检在增生期或月经周期分泌期的中间阶段进行。通过使用单克隆抗体-Bond Ready-to-Use Primary Antibody CD68对狭缝染色,对获得的生物材料进行免疫组织化学研究。通过计数1 mm 2 的阳性细胞数来确定CD68巨噬细胞的数量。如果子宫内膜CD68阳性染色的细胞数在增殖的中间阶段(从151到293)或分泌期的中间阶段(从425到523)为1 mm 2 ,则可诊断为慢性子宫内膜炎。如果在增殖期的中间阶段(从49到150)或分泌期的中间阶段(从74到424),子宫内膜CD68阳性染色细胞的数量为1 mm 2 效果:本发明为患有子宫内膜异位症的女性提供了慢性子宫内膜炎的有效诊断方法。1cl,3 dwg,4 ex

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