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Method for constructing GLRX1 gene knock-out animal model based on crispr/cas9
Method for constructing GLRX1 gene knock-out animal model based on crispr/cas9
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机译:基于crisper / cas9的GLRX1基因敲除动物模型的构建方法
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摘要
The invention discloses a method for constructing a Glrx1 gene knock-out animal model based on CRISPR/Cas9, comprising the steps of: 1. selection and design of sgRNA targeting Glrx1 gene of mice; 2. construction of an sgRNA vector; 3. in vitro transcription of sgRNA; 4. injection of mouse fertilized eggs; 5. birth and identification of F0-generation mice; and 6. breeding of positive F0-generation mice, and birth and identification of F1-generation mice.
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