Virion display array for profiling the function and interaction of human membrane proteins

摘要

Provided herein are recombinant virion arrays, recombinant HSV-1 virions, and methods of use that include human membrane-bound proteins that retain their native conformation and / or interaction, to human membrane-bound proteins. Includes high content, high throughput assays, diagnostic assays, proteome assays, and biosensor assays for screening for binding ligands and / or drugs. Recombinant HSV-1 virions containing an envelope containing a plurality of heterologous membrane-binding proteins that retain their unique conformation and / or interaction, as well as recombinant HSV-1 bacterial artificial chromosomes that encode heterologous membrane polypeptides ( BAC) clones are also provided.