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用于鉴定黄瓜黑斑病抗性的引物序列及其鉴定方法

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摘要

本发明公开一种用于鉴定黄瓜黑斑病抗性的引物序列及其鉴定方法，引物序列是由序列表中SEQ ID No1、SEQ ID No2所述的核苷酸序列组成；鉴定黄瓜黑斑病抗性的方法，步骤是(1)提取黄瓜基因组DNA；(2)用序列表中SEQ ID No1、SEQ ID No2所述的核苷酸序列为引物进行PCR扩增；(3)扩增产物的凝胶电泳分析；(4)依据各被鉴定样品在凝胶上条带的相对位置，鉴定黄瓜黑斑病的抗性。利用本发明的引物序列对构建的F

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公开/公告号CN101845504B

专利类型发明专利
公开/公告日2012-06-27

原文格式PDF
申请/专利权人天津科润农业科技股份有限公司;
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申请/专利号CN201010197951.4
发明设计人李淑菊;王惠哲;杨瑞环;管炜;
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申请日2010-06-11
分类号
代理机构天津市北洋有限责任专利代理事务所;
代理人陆艺
地址 300457 天津市经济技术开发区第四大街80号天大科技园A2座8层
入库时间 2022-08-23 09:10:25

法律信息

法律状态公告日

法律状态信息

法律状态
2016-08-03

未缴年费专利权终止 IPC(主分类):C12Q 1/68 授权公告日:20120627 终止日期:20150611 申请日:20100611

专利权的终止
2012-06-27

授权

授权
2010-11-17

实质审查的生效 IPC(主分类):C12Q 1/68 申请日:20100611

实质审查的生效
2010-09-29

公开

公开

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1. 用于鉴定黄瓜黑斑病抗性的引物序列及其鉴定方法 [P] . 中国专利： CN101845504B . 2012.06.27
2. 用于鉴定黄瓜炭疽病抗性的引物序列及其鉴定方法 [P] . 中国专利： CN100374580C . 2008.03.12
3. Nucleotide sequences of the mutant and non-mutant fragments of loci A and C of alleles of double low (00) fad3 form desaturase gene and low-linolenic mutant (LLMut) of oilseed rape plants (Brassica napus L. var. Oleifera), allospecific SNP markers specific for loci A and C of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLMut) of winter oilseed rape plants, nucleotide sequences of primer pairs for the amplification of loci A and C of fad3 form desaturase gene of winter oilseed rape plants, method for amplification of loci A and C of fad3 desaturase gene of winter oilseed rape plants, nucleotide sequences of the primers for the identification of mutant and non-mutant alleles of fad3 forms desaturase gene - double low (00) and low-linolenic mutant (LLMut) of oilseed rape in locus A and locus C in the microsequencing reaction, method for the identification of mutant and non-mutant alleles of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLM [P] . PL211126B1 . 2012-04-30

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5. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

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