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一种咖啡果小蠹的特异性SS-COI引物对、鉴定试剂盒和快速鉴定方法

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摘要

本发明涉及生物技术领域，特别涉及一种咖啡果小蠹的特异性SS‑COI引物对、鉴定试剂盒和快速鉴定方法。该特异性SS‑COI引物对由SEQ ID NO：1所示的上游引物和SEQ ID NO：2所示的下游引物组成。本发明特异性SS‑COI引物和方法实现了对咖啡果小蠹的快速分子鉴定，方法简单，便于操作，耗时短；鉴定范围广、灵敏度高。

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公开/公告号CN111826450B

专利类型发明专利
公开/公告日2021-08-24

原文格式PDF
申请/专利权人中国热带农业科学院香料饮料研究所;
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申请/专利号CN202010920559.1
发明设计人王政;孟倩倩;孙世伟;刘爱勤;苟亚峰;高圣风;
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申请日2020-09-04
分类号C12Q1/6888(20180101);C12N15/11(20060101);
代理机构11227 北京集佳知识产权代理有限公司;
代理人潘颖
地址 571533 海南省万宁市兴隆热带植物园
入库时间 2022-08-23 12:21:10

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1. 一种咖啡果小蠹的特异性SS-COI引物对、鉴定试剂盒和快速鉴定方法 [P] . 中国专利： CN111826450B . 2021.08.24
2. 一种快速鉴定果园高效解磷细菌的特异性引物及其试剂盒、分离鉴定方法 [P] . 中国专利： CN110592249A . 2019-12-20
3. Processes for the identification of an elite event in biological samples, for the confirmation of the purity of seed, for the selection of seeds in the presence of said event elite.For the determination of the status of zigosidade of a plant, material of plant or seed which comprises the said event elite, to detect the presence of said event elite.And for the production of a plant or a seed of cotton which comprises the said event elite, as well as kits for the identification of said event elite.A pair of primers suitable for use in specific detection of the same, and specific probe for the identification of said event elite [P] . BRPI0614338B1 . 2018-01-02

机译：用于鉴定生物样品中优良事件，确认种子纯度，在存在所述优良事件的情况下选择种子的方法。用于确定植物，植物或植物的齐哥状态的状态包括所述事件精英的种子，以检测所述事件精英的存在。并且用于生产包含所述事件精英的植物或棉花种子，以及用于鉴定所述事件精英的试剂盒。适用于特异性检测的引物的鉴定以及用于鉴定所述事件精英的特异性探针
4. Nucleotide sequences of primers specific for the terminal areas of the tested DNA fragment conjugated with Rfo restorer gene for CMS ogura in rape, method for DNA amplification by polymerase chain reaction (PCR) with primers specific for genomic DNA fragment coupled with Rfo gene for CMS ogura in rape, process for the preparation of the nucleotide sequence of the DNA fragment conjugated the Rfo restorer gene for CMS ogura in rape, process for the preparation of SCAR type DNA marker for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility, method for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility and test kit for the identification of rape plants with Rfo restorer gene for ogura gene-cytoplasmic male sterility [P] . PL212433B1 . 2012-10-31

机译：油菜中与CMS ogura的Rfo恢复基因偶联的被测DNA片段末端区域特异的引物的核苷酸序列，通过基因组DNA片段的特异性引物与CMS ogura的Rfo基因偶联的聚合酶链反应（PCR）扩增DNA的方法在油菜中，制备与油菜CMS ogura的Rfo恢复子基因偶联的DNA片段的核苷酸序列的方法，在SCAR型DNA标记物的制备方法中用于鉴定含ogura基因的Rfo恢复子基因的油菜植物雄性不育，带有Rfo恢复基因的油菜植株的ogura基因胞质雄性不育的鉴定方法和试剂盒
5. Control primer annealing to improve the specificity of annealing in nucleic acid amplification kit.Methods for amplifying a nucleic acid sequence of a DNA or a mixture of nucleic acids.And for selectively amplifying a nucleic acid sequence of a target DNA or a mixture of nucleic acids, and a sequence of the target nucleic acid from a mRNA.Method for detecting the complementarity of DNA to mRNA differentially expressed in two or more samples of nucleic acid, methods for amplifying a fragment of the cDNA and DNA quickly target.And to amplify a population of cDNAs of filament double full-length complementary to mRNAs cDNAs, and filament double fortified with 5 'complementary to mRNAs,Method to amplify rather than a sequence of nucleotu00ecdeo target at the same time, methods for producing a digital printing gDNA DNA, and RNA in a sample of mRNA.Method for identifying segments of homology is conserved in a family of multigene from a sample of mRNA, a method for the mutagenesis in a target [P] . BR0214741A . 2004-11-23

机译：控制引物退火，以提高核酸扩增试剂盒中退火的特异性。扩增DNA或核酸混合物的核酸序列的方法以及选择性扩增靶DNA或核酸混合物的核酸序列的方法用于检测DNA与两个或多个核酸样品中差异表达的mRNA的互补性的方法，扩增cDNA和DNA片段的方法可快速靶向。全长与mRNA cDNA互补的双细丝cDNA的cDNA群体，以及与mRNA互补的5'增强双细丝的cDNA，同时扩增而不是核苷酸靶序列的方法，产生数字印刷gDNA DNA的方法mRNA样品中的RNA和RNA.mRNA样品的多基因家族中用于鉴定同源片段的方法是保守的，这是一种在靶标中诱变的方法