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鉴定皂荚性别的基因组合、SSR引物组合、鉴定方法和应用

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摘要

本发明提供了鉴定皂荚性别的基因组合、SSR引物组合、鉴定方法和应用，涉及分子生物学DNA分子标记检测，所述基因组合包括G1基因、G2基因、G3基因、G4基因和G5基因；所述G1～5基因依次具有SEQ ID No.1～5所示的核苷酸序列。采用本发明提供的基因组合能够鉴定皂荚的性别，准确率为100％。

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公开/公告号CN109457023B

专利类型发明专利
公开/公告日2021-07-13

原文格式PDF
申请/专利权人河南师范大学;
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申请/专利号CN201811609308.0
发明设计人李建军;叶承霖;
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申请日2018-12-27
分类号C12Q1/6879(20180101);C12Q1/6895(20180101);C12N15/11(20060101);
代理机构11569 北京高沃律师事务所;
代理人代芳
地址 453000 河南省新乡市牧野区建设东路46号
入库时间 2022-08-23 12:07:43

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3. Nucleotide sequences of the mutant and non-mutant fragments of loci A and C of alleles of double low (00) fad3 form desaturase gene and low-linolenic mutant (LLMut) of oilseed rape plants (Brassica napus L. var. Oleifera), allospecific SNP markers specific for loci A and C of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLMut) of winter oilseed rape plants, nucleotide sequences of primer pairs for the amplification of loci A and C of fad3 form desaturase gene of winter oilseed rape plants, method for amplification of loci A and C of fad3 desaturase gene of winter oilseed rape plants, nucleotide sequences of the primers for the identification of mutant and non-mutant alleles of fad3 forms desaturase gene - double low (00) and low-linolenic mutant (LLMut) of oilseed rape in locus A and locus C in the microsequencing reaction, method for the identification of mutant and non-mutant alleles of fad3 form desaturase gene - double low (00) and low-linolenic mutant (LLM [P] . PL211126B1 . 2012-04-30

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