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一种用于检测血清中VEGF含量的抗体对及其用途

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摘要

本申请提供一种用于检测血清中VEGF含量的抗体对及其用途，包括与VEGF的N端表位即SEQ ID NO:2所示氨基酸序列相结合的单克隆抗体AntiVEGF_N和与VEGF的C端表位即SEQ ID NO:3所示氨基酸序列相结合的单克隆抗体AntiVEGF_C，所述单克隆抗体AntiVEGF_N包括SEQ ID NO:7所示氨基酸序列的轻链可变区和SEQ ID NO:11所示氨基酸序列的重链可变区，所述AntiVEGF_C包括SEQ ID NO:17所示氨基酸序列的轻链可变区和SEQ ID NO:21所示氨基酸序列的重链可变区。本申请将VEGF抗原分成N端(SEQ ID NO:2)和C端(SEQ ID NO:3)两段序列，并用这两段序列分别免疫小鼠，筛选到高特异性和高灵敏性的抗体对AntiVEGF_N和AntiVEGF_C，通过该抗体对可以有效的测定血清中VEGF的含量，进而便于肿瘤广谱及早期筛查，大大提高了肿瘤早期筛查的准确性。

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公开/公告号CN111808191A

专利类型发明专利
公开/公告日2020-10-23

原文格式PDF
申请/专利权人廊坊天光生物技术有限公司;
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申请/专利号CN202010389678.9
发明设计人刘继来;刘静;程勇;王征;
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申请日2020-05-11
分类号C07K16/28(20060101);C12N15/13(20060101);G01N33/74(20060101);G01N33/574(20060101);
代理机构11435 北京志霖恒远知识产权代理事务所(普通合伙);
代理人韩亚伟
地址 065500 河北省廊坊市固安县新兴产业示范园区肽谷生物医药产业园孵化楼
入库时间 2023-06-19 08:36:28

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