Untersuchungen zur enzymatischen Bleiche von kanariengelber Wolle während der Rohwollwäsche und Charakterisierung der Wollproteine nach Einwirkung von Enzymen

摘要

It is the aim of this theses to investigate the possibility to bleach wool with several enzymes during the raw wool scouring process. Special emphasis lies on the bleaching of canary yellow wool and the investigation of the enzyme's influence on the morphology of the woolfibre. The bleaching of wool with horseradish-peroxidases and hydrogenperoxide showed that the achieved whitening was only due to the latter one. The enzymatic treatment with different Subtilisin-proteases () showed, that a whitening effect can be achieved, which is mainly due to the incubation time of the enzyme does not depend very much on the enzyme concentration. SEM pictures show that the incubation of wool fibres with SP 655 - even with long incubation times - has only little effect on the single fibre whereas the SEM pictures of woolfibres incubated with the other two proteases show some fibres where the whole fibre compound is badly damaged. Despite of this the separation pattern of the extractable woolproteins show no significant change of enzyme treated or untreated wool samples. The investigation of woolfibre-crosssections of samples treated with proteases by TEM show a successive degradation of the cuticle, endocuticle, the cell-membrane-complex and the cortex. Further investigations of these samples with the help of SDS-PAGE and IEF show a successive degradation of woolproteins from the higher molecular ones down to small, lower molecular proetin fragments which is due to the influence of the proteases.