Use of Gene Probes in the Rapid Analysis of Natural Microbial Communities

摘要

Hybridization probes produced from DNA sequences have proven to be a powerful tool in rapid and sensitive analysis of natural microbial communities. By using function-specific probes, such as those identifying genes coding for photosynthesis, the potential a microbial community has for performing a given function may be rapidly determined. Gene probes have also been used in the identification and isolation of a specific catabolic genotype in less than one-fourth the time required for the conventional culture enrichment technique. Species specific probes constructed from portions of genes coding for ribosomal RNA have been used for the rapid identification and enumeration of bacterial species in environmental samples. The use of reassociation kinetics as a measure of community diversity and complexity is also discussed. The successful application of this technique to community analysis may reduce the time required from one year, for conventional analysis, to two weeks. (Copyright (c) 1988 Society for Industrial Microbiology.)