Development and Application of Subtractive Hybridization Strategies to Facilitate211 Gene Discovery. Final report

摘要

We have developed and applied subtractive hybridization strategies to generate211u001ecDNA libraries enriched for novel cDNAs. Pools of I..M..A.G.E clones, from which 211u001eESTs have been derived, are used as drivers in hybridizations with single or 211u001emultiple normalized libraries thus generating subtracted libraries enriched for 211u001ecDNAs not yet represented in public databases. Subtracted libraries are 211u001echaracterized by Southern hybridization to assess reduction in representation of 211u001eclones of the driver population and then contributed to the I.M.A.G.E. consortium 211u001efor large-scale arraying and sequencing.