High-resolution Fluorescence Spectroscopy in Immunoanalysis

摘要

The work presented in this dissertation combines highly sensitive and selective fluorescence line-narrowing spectroscopy (FLNS) detection with various modes of immunoanatytical techniques. It has been shown that FLNS is capable of directly probing molecules immunocomplexed with antibodies, eliminating analytical ambiguities that may arise from interferences that accompany traditional immunochemical techniques. Moreover, the utilization of highly cross-reactive antibodies for highly specific analyte determination has been demonstrated. Finally, we demonstrate the first example of the spectral resolution of diastereomeric analytes based on their interaction with a crossreactive antibody.