Evaluation of Triethanolamine 80/475 in the Primary Rat Hepatocyte Unscheduled Dna Synthesis Assay (final Report) with Cover Sheet dated 061289.

摘要

Genotoxicity of triethanolamine was evaluated in the rat hepatocyte primary culture/DNA repair assay. The compound was tested at concentrations of 0.5x10E-8, 1x10E-8, 2.5x10E-8, 0.5x10E- 7, 1x10E-7, 2.5x10E-7, 0.5x10E-6, and 1x10E-6 M, in Williams Medium E with 10% fetal calf serum. The negative control and positive control groups were cell cultures exposed to the medium only, and the medium plus 0.05 ug/ml 2-acetylaminofluorene, respectively. The treatment was cytotoxic to the cell cultures at 1x10E-6. The treatment had no effect on the rate of DNA repair, indicating a lack of genotoxicity of triethanolamine in this cell culture assay.