CDNA Fingerpinting of Breast Cancer Tumor Cells

摘要

This research is developing and applying comparative DNA analysis to breast cancer disease. For instance, methods for making DNA chip arrays useful for matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis were developed. Targeted genomic and cDNA differential display (TGOD and TcDD respectively) will be used to feed sample hungry MS instruments pools of fragments containing a common sequence (e. g. (CAG)n, or other repeat sequences, LTR sequences (retroviral footprints), or Zn-finger binding motifs (transcription factor coding sequences)). Targetings is on important dispersed gene and sequence families whose expresson, or genomic organization may be modified in tumor cells. Other array oriented technology developed include rolling circle amplification (RCR: an in situ isothermal DNA amplification) and an in situ scoring method for genetic markers. Cloneless libraries generated from restriction enzyme cleaved genomic DNA fractionated by electrophoresis were used to characterize the chromosome 20q1 3 region amplified in breast and ovarian cells, and to identify 70 cDNAs from this region. Gel lane slices containing DNA constitute the library.