Autologous Tumor Vaccination

摘要

The concept was to implant, subcutaneously, breast cancer cells in syngeneic rats, inject immune system stimulators entrapped in a sustained release gel into the tumor and observe a short term and a long term immune response to the tumor. Tumors appeared about 8 days after injecting 1,000,000 cells. As soon as we injected 100 ug of N-formyl-Met-Leu-Phe and 0.1 ug of IL-12 in 0.3 ml of gel, an anti-tumor immune response ensued. Tumor growth halted for about 4 days and then resumed at the rate of untreated control cells. If the tumor was re-injected a second and then a third time, tumor growth halted and resumed. However, 3 consecutive injections was not sufficient to induce a long term response. These results were reproducible and encouraging, considering it was a first attempt. Either stimulant alone or both in combination gave the same results. If continued, we would have to develop a gel formulation that would release the stimulants more slowly than the half- time of about 1 day with the present gel.