Angiogenic Signaling in Living Breast Tumor Models

摘要

In this grant we proposed to elucidate the signaling pathway that translates VEGFR activation into elevated vessel permeability, in endothelial cells within living breast tumor models. The working hypothesis is that the signaling pathway involved is a constitutively active form of the pathway shown for healthy mesenteric microvessels. In parallel we proposed to develop relevant optical techniques including Multiphoton Fluorescence Recovery After Photobleaching in vivo and Second Harmonic Generation (SHG) imaging. Progress to date includes the training of personnel in the laboratory, the addition to MPFRAP of the ability to measure diffusion in the presence of nearby obstacles, the elucidation of the role of the b-AR signaling pathway in breast tumor models, the elucidation of the role of PLCg in tumor endothelial cell VEGF response, and the creation of the ability to measure the forwards/backwards scattering ratio of SHG emission from collagen.