PROPERTIES OF CULTURES OF ANTHRAX BACILLI GROWN AT VARIOUS TEMPERATURES

摘要

1. When incubating a culture of anthrax bacilli at 43 we observed the formation of spores in them. Therefore, in spite of the generally acknowledged opinion, established in the literature since the time of Tsenkovskiy, we cannot consider incubation of anthrax cultures at the stated temperature as. a. completely satisfactory method for obtaining anthrax strains.n2. By incubating a. culture of anthrax bacilli in meat-peptone chicken broth with a pH = 7.1 - 7.2 at 45°, we established that under these conditions they do not transform from the vegetative form into the spore form. During this the morphological, cultural and biochemical properties of the culture did not change.n3. In a culture obtained following incubation of a virulent strain of the anthrax causative agent in broth at 45° for 7 days, a low virulence was combined with high immunizing properties -- after a single inoculation an intense immunity set-in in the animals.