Histochemistry of Renal Lesions Induced by Pulsatile Hypothermic Perfusion.

摘要

Two groups of 10 and 11 canine kidneys were perfused with pulsatile flow at 25 and 5 C., respectively. Marked microscopic quantitative and qualitative differences between the two groups of perfused kidneys were noted, particularly with respect to the glomerular lesions. In the 5 C. perfusion group, 79.1% of the counted glomeruli were structurally intact, whereas only 43.2% were intact following 25 C. perfusion. The glomeruli of kidneys perfused at 25 C. were frequently infarcted (30.0%). The acellular material deposited in infarcted glomeruli contained protein, acid and neutral polysaccharides, and RNA, but no amyloid. Tubular cells of both series contained very little RNA and almost invariably exhibited a strong false positive Feulgen reaction. Hydropic vacuolation was noted in the proximal and distal tubules of both series. Cytoplasmic inclusions usually associated with proteinuria prevailed only in the 25 C. perfusion group. Intravascular depositions of the kidneys perfused at 25 C. frequently contained RNA. It is concluded that hypothermic pulsatile perfusion, apart from inducing purely morphologic alterations, also induces pronounced temperature-dependent changes in distribution and reactivity of histochemically demonstrable renal nucleic acids, carbohydrates, and protein.