Plasmid Isolation in Legionella pneumophila and Legionella-like Organisms

摘要

Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid. Cryptic plasmids were found in three of the isolates examined. A purification protocol was devised which consolidates the central tenets of several existing isolation techniques into a single procedure. Plasmid molecular weights were determined to range from 46.6 to 59.8 megadaltons (Mdal). (Author)