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Effect of Chemical Mutagens on Herpes Virus-Induced Cellular Transformation and Testing for Mutagenesis in Mouse Cells

机译:化学诱变剂对疱疹病毒诱导的细胞转化和小鼠细胞诱变检测的影响

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Assays which detect quantitative increases of morphological transformation in 3T3 cells were employed to detect transformation by seven temperature sensitive (ts) mutants of herpes simplex type 2 virus (HSV-2). It was determined that all seven mutants caused transformation at the non-permissive temperature. Mutant A8 (293) caused formation of the most foci and appeared to be a more sensitive indicator of transformation in comparative experiments (31 foci per million cells compared to four foci per million cells for ultraviolet-irradiated wild type virus). (Author)

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