Effect of Chemical Mutagens on Herpes Virus-Induced Cellular Transformation and Testing for Mutagenesis in Mouse Cells.

摘要

Assays which detect quantitative increases of morphological transformation in 3T3 cells were employed to detect transformation by seven temperature sensitive (ts) mutants of herpes simplex type 2 virus (HSV-2). It was determined that all seven mutants caused transformation at the non-permissive temperature. Mutant A8 (293) caused formation of the most foci and appeared to be a more sensitive indicator of transformation in comparative experiments (31 foci per million cells compared to four foci per million cells for ultraviolet-irradiated wild type virus). (Author)