Comprehensive Package for DNA Sequence Analysis in FORTRAN IV for the PDP-11

摘要

A computer package written in Fortran-IV for the PDP-11 minicomputer is descibed. The package's novel features are: (i) software for voice-entry of sequence data; (ii) a less memory intensive algoithm for optimal sequence alignment; (iii) and programs that fit statistical models to nucleic acid and protein sequences. The widespread use of rapid methods for DNA sequence analysis (32, 41) and the availability of over five thousand published DNA sequences in computerized data bases, such as GENBANK (TM), has mandated the development of computer packages for the collection (36), organization, and statistical analysis of these nucleic acid sequences (44, 45, 46, 58). We present one such package named DNASEQ developed at the University of Georgia. The package contains several novel features which include: (i) software for the voice entry of nucleic acid or protein sequence data; (ii) a less memory-intensive version of the Needleman and Wunsch (34) algorithm for sequence alignment; and (iii) programs that fit statistical models to nucleic acid and protein sequences.