Multicomponent Analysis Using Synchronous-Excitation Phase-Resolved Fluorescence Spectroscopy

摘要

Phase resolved fluorescence spectroscopy was used to incorporate fluorescence lifetime selectivity into multicomponent fluorimetric determinations. Quantitative analysis was demonstrated for systems of up to six fluorescent components with overlapping spectra. Multidimensional data formats of phase resolved fluorescence intensity as a function of spectral and lifetime parameters were developed, including formats for both synchronous excitation and total luminescence spectroscopy. It was shown that phase resolution improves quantitative results significantly over the use of steady-state, non-lifetime dependent intensity measurements. Specific systems studied include polycyclic aromatic hydrocarbons and metal chelates. Sodium taurocholate micelles were studied as a means by which synergistic interactions between fluorescence molecules in a sample could be minimized. Very interesting results were obtained, and further investigations of the micellar systems are being conducted.