Multicomponent Analysis by Submillimeter-Effective- Length Capillary Electrophoresis using Patterned Fluorescence Correlation Spectroscopy

摘要

A multicomponent system is analyzed by using a combination of capillary electrophoresis and patterned fluorescence correlation Spectroscopy. In this combination, fluorescent analyte molecules dispersed continuously in a capillary migrate through a stationary interference pattern created by two intersecting excitation laser beams, fluorescence emission from which is monitored with a single detector. The power spectrum of fluctuations in the detector photocurrent gives a virtual electropherogram. Using an effective length of 740 μm, we obtained a resolution of 1.0 for a two-component injection of TOTO-1-stained pUC18 DNA and λ DNA.