Comparative Infectivity Determinations of Dengue Virus Vaccine Candidates inRhesus Monkeys, Mosquitoes, and Cell Cultures

摘要

Attenuation of virulent viruses are widely used for the development of viralvaccines through the years. These vaccines are made by serial passages of virus in cells from non-natural hosts. The first intents for the development of Dengue virus vaccines was made by serial intracerebral passages of virus in infant of newly-weaned mice. Primary canine kidney (PCK) cell culture are also used for the attenuation of wild Dengue virus strains. Studies using these attenuated virus strains have been recently published. The objective of this contract was to determine the antibody response of rhesus macaques inoculated with different attenuated dengue virus preparations. Vaccine immunogenecity was determined by the plaque reduction neutralization test (PRNT). The PRNT titer of each serum sample was determined as described elsewhere.