Mechanism(s) of Electricity Production by Shewanella and other Microbes: Understanding and Optimization.

摘要

In the five years of this MURI program, we have made major advances in several areas, including: (1) power production optimization; (2) MFC design; (3) identification of the genes coding for the proteins involved with electricity production by Shewanella oneidensis (MR-1); (4) construction of conceptual models of extracellular electron transport (EET); (5) characterization and quantification of the per cell rates of EET to solid substrates; (6) characterization of a previously undescribed behavioral adaptation of microbes to charged surfaces called electrokinesis (and the impact of surface charge on bacterial attachment and biofilm formation); (7) the use of Vertical Scanning Interferometry for cell and biofilm analyses; (8) the design and implementation of a new type of (Deep Ultraviolet Light) microscope for non-invasive studies of microbes on surfaces; (9) detailed biophysical studies of the mechanism of electron transfer in conductive appendages called bacterial nanowires, produced by MR-1 and other Shewanella strains and species; (10) characterization of the selective advantages of Shewanella cells in late stationary phase, and the relationship of this metabolic state to the long term function of MFC systems; and, (11) characterization of the activities of microbes acting as catalysts on the cathodes of MFC systems. During this time, we published over *** reviewed papers, presented over 100 talks at scientific meetings, and collaborated with colleagues from around the world.