In Vivo Transcriptional Regulation of the Human HMG-1/Y Gene as it Relates to theProgression of Breast Cancer

摘要

Increased HMG (high mobility group)-I;/Y expression is correlated with moreadvanced cancers and with high metastatic potential. To determine what regulates the human HMG-I/Y gene in breast cancer, we studied the expression of the HMG-I/Y gene in two human breast cancer cell lines: MCVI cells which are estrogen receptor positive and Hs578T cells which are estrogen receptor negative. HMG-I/Y expression was studied in the presence and absence of b-estradiol and epidermal growth factor (EGF). No increase in HMG-I/Y messenger RNA was observed in b-estradiol or BGF treated MCF7 cells. In contrast, in the more metastatic Hs578T cell line there is a dramatic increase in HMG-bY mRNA expression, up to twenty-three fold, when treated with EGF. HMG-I/Y mRNA expression was further characterized in the EGF treated 11s578T cells using primer extensions, which showed that two of the multiple possible HMG-I/Y transcripts are induced. HMG-I/Y